All of the information which is here came from other websites. The answers are correct as far as I know. If you find anything that seems incorrect, I would appreciate hearing about it so that I can make corrections.
| Purpose of Staining: increases Contrast & Resolution | Provide contrast (easier to see cells against background (slide) | |||||||||||||
| Simple Stain | 1 stain used: Basic (OH-) or acidic (H+) (BOOK -ONLY BASIC DYES) | NO HEAT SETTING- Use dye 30-60 sec and rinse | ||||||||||||
| Basic Stain | (+) Cationic chromophores | Colored part has a (+) charge: Methylene blue(+) and chloride(-) |
stain acidic structures / best under basic high Ph(Most cells have a natural (–) negative charge à Attract Stain)
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| BASIC stain colors à | ||||||||||||||
| Direct Stain | Simple Procedure – BASIC stains used / Stain organism Directly | |||||||||||||
| Acidic Stain | (-) Anionic Chromophores | Colored part Negative (-) charge (stain repelled by cells) | Stain alkaline structures / work best in acidic low Ph | Background (Slide) is stained / cells remain CLEAR
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| Acidic stain colorsà | ||||||||||||||
| Negative Stain | NO heat fixing | Acidic Stains Used: | 1. colors background | 2. Leaves cells clear | ||||||||||
| Capsule staining: | + Positive stain colors the body | – Negative stain colors the background | Treponema pallidum – will not stain directly | Causative agent of syphilis | ||||||||||