{"id":532,"date":"2018-07-21T10:05:11","date_gmt":"2018-07-21T15:05:11","guid":{"rendered":"http:\/\/myallaboutyou.com\/?page_id=532"},"modified":"2026-08-25T07:34:42","modified_gmt":"2026-08-25T12:34:42","slug":"microbiology-lab-231","status":"publish","type":"page","link":"https:\/\/my-all-about-you.com\/en\/reference\/microbiology-lab-231\/","title":{"rendered":"Microbiology Lab"},"content":{"rendered":"\n
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A note about these archived materials<\/h2>\n\n\n

This page preserves study notes prepared during earlier coursework. It has not been comprehensively revised to reflect current clinical, scientific, testing, or regulatory standards. It is provided for educational and historical reference only\u2014not as medical advice, a treatment guide, or a substitute for current course materials and authoritative health guidance.<\/p>\n\n\n

Return to The Study<\/a>  |  Visit Body & Grace<\/a><\/p>\n<\/div>\n\n\nAll of the information which is here came from other websites.  The answers are correct as far as I know.  If you find anything that seems incorrect, I would appreciate hearing about it so that I can make corrections.\n\n\n\n\n\n
Purpose of Staining<\/strong>:  increases Contrast & Resolution<\/strong>\n\n<\/td>\n<\/td>\n \n\n<\/td>\nProvide contrast <\/strong>(easier to see cells against background (slide)\n\n<\/td>\n<\/tr>\n
Simple Stain<\/strong><\/td>\n1<\/strong> stain used: Basic<\/strong> (OH-)<\/strong> or acidic (H+) (BOOK -ONLY BASIC DYES)<\/strong>\n\n<\/td>\n<\/td>\nNO HEAT SETTING- <\/strong>Use dye 30-60 sec and rinse\n\n<\/td>\n<\/td>\n<\/tr>\n
Basic <\/strong>Stain<\/strong>\n\n<\/td>\n<\/td>\n <\/strong>\n\n<\/td>\n(+) Cationic chromophores\n\n<\/td>\nColored part has a (+) charge:  Methylene blue(+) and  chloride(-)\n\n<\/td>\n<\/td>\n\n\n\n\n
\n\n\n\n
Crystal violet<\/strong> stains cells NOT CAPSULES<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>stain acidic structures \/ best under basic<\/em> high Ph(Most cells<\/strong> have a natural (\u2013) negative charge<\/strong> \u00e0 Attract<\/strong> Stain<\/strong>)\n\n\n\n\n\n\n\n\n\n\n
<\/td>\n<\/tr>\n
<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table> \n\n \n\n<\/td>\n<\/tr>\n
BASIC stain colors <\/strong>\u00e0<\/td>\n<\/tr>\n
Direct Stain<\/strong><\/td>\nSimple Procedure \u2013 BASIC<\/strong> stains used \/ Stain organism<\/strong> Directly<\/strong><\/td>\n<\/tr>\n
Acidic <\/strong>Stain<\/strong>\n\n<\/td>\n<\/td>\n \n\n<\/td>\n(-) Anionic Chromophores\n\n<\/td>\nColored part Negative (-) charge (stain repelled<\/strong> by cells)\n\n<\/td>\n<\/td>\nStain alkaline structures \/ work best in acidic<\/em> low Ph\n\n<\/td>\nBackground (Slide) is stained<\/strong> \/ cells remain CLEAR<\/strong>\n\n \n\n \n\n \n\n \n\n \n\n \n\n<\/td>\n<\/tr>\n
\nAcidic stain colors<\/strong>\u00e0<\/strong><\/td>\n<\/tr>\n
Negative<\/strong> Stain<\/strong>\n\n<\/td>\n<\/td>\n \n\n<\/td>\nNO heat fixing<\/strong>\n\n<\/td>\nAcidic Stains Used<\/strong>:\n\n<\/td>\n<\/td>\n1.        colors background\n\n<\/td>\n2.       Leaves cells clear\n\n<\/td>\n<\/tr>\n
Capsule staining: <\/strong>\n\n<\/td>\n<\/td>\n+ Positive stain colors the body\n\n<\/td>\n– Negative stain colors the background\n\n<\/td>\nTreponema pallidum<\/em> \u2013 will not stain directly\n\n<\/td>\n<\/td>\nCausative agent of syphilis\n\n<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>","protected":false},"excerpt":{"rendered":"

A note about these archived materials This page preserves study notes prepared during earlier coursework. It has not been comprehensively revised to reflect current clinical, scientific, testing, or regulatory standards. It is provided for educational and historical reference only\u2014not as medical advice, a treatment guide, or a substitute for current course materials and authoritative health […]<\/p>\n","protected":false},"author":132804860,"featured_media":533,"parent":73,"menu_order":5,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_coblocks_attr":"","_coblocks_dimensions":"","_coblocks_responsive_height":"","_coblocks_accordion_ie_support":"","_wpcom_ai_launchpad_about_page":false,"_wpcom_ai_launchpad_gallery_page":false,"_wpcom_ai_launchpad_contact_page":false,"_wpcom_ai_launchpad_events_page":false,"_wpcom_ai_launchpad_video_page":false,"_wpcom_ai_launchpad_portfolio_piece":false,"footnotes":""},"class_list":["post-532","page","type-page","status-publish","has-post-thumbnail","hentry"],"jetpack_likes_enabled":true,"jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/P9vUsN-8A","jetpack-related-posts":[],"_links":{"self":[{"href":"https:\/\/my-all-about-you.com\/en\/wp-json\/wp\/v2\/pages\/532","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/my-all-about-you.com\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/my-all-about-you.com\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/my-all-about-you.com\/en\/wp-json\/wp\/v2\/users\/132804860"}],"replies":[{"embeddable":true,"href":"https:\/\/my-all-about-you.com\/en\/wp-json\/wp\/v2\/comments?post=532"}],"version-history":[{"count":5,"href":"https:\/\/my-all-about-you.com\/en\/wp-json\/wp\/v2\/pages\/532\/revisions"}],"predecessor-version":[{"id":16195,"href":"https:\/\/my-all-about-you.com\/en\/wp-json\/wp\/v2\/pages\/532\/revisions\/16195"}],"up":[{"embeddable":true,"href":"https:\/\/my-all-about-you.com\/en\/wp-json\/wp\/v2\/pages\/73"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/my-all-about-you.com\/en\/wp-json\/wp\/v2\/media\/533"}],"wp:attachment":[{"href":"https:\/\/my-all-about-you.com\/en\/wp-json\/wp\/v2\/media?parent=532"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}