{"id":532,"date":"2018-07-21T10:05:11","date_gmt":"2018-07-21T15:05:11","guid":{"rendered":"http:\/\/myallaboutyou.com\/?page_id=532"},"modified":"2025-09-21T15:39:11","modified_gmt":"2025-09-21T20:39:11","slug":"microbiology-lab-231","status":"publish","type":"page","link":"https:\/\/my-all-about-you.com\/en\/reference\/microbiology-lab-231\/","title":{"rendered":"Microbiology Lab"},"content":{"rendered":"

All of the information which is here came from other websites.  The answers are correct as far as I know.  If you find anything that seems incorrect, I would appreciate hearing about it so that I can make corrections.<\/p>\n\n\n\n\n\n
Purpose of Staining<\/strong>:  increases Contrast & Resolution<\/strong><\/p>\n<\/td>\n<\/td>\n <\/p>\n<\/td>\nProvide contrast <\/strong>(easier to see cells against background (slide)<\/p>\n<\/td>\n<\/tr>\n
Simple Stain<\/strong><\/td>\n1<\/strong> stain used: Basic<\/strong> (OH-)<\/strong> or acidic (H+) (BOOK -ONLY BASIC DYES)<\/strong><\/p>\n<\/td>\n<\/td>\nNO HEAT SETTING- <\/strong>Use dye 30-60 sec and rinse<\/p>\n<\/td>\n<\/td>\n<\/tr>\n
Basic <\/strong>Stain<\/strong><\/p>\n<\/td>\n<\/td>\n <\/strong><\/p>\n<\/td>\n(+) Cationic chromophores<\/p>\n<\/td>\nColored part has a (+) charge:  Methylene blue(+) and  chloride(-)<\/p>\n<\/td>\n<\/td>\n\n\n\n\n
\n\n\n\n
Crystal violet<\/strong> stains cells NOT CAPSULES<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

stain acidic structures \/ best under basic<\/em> high Ph(Most cells<\/strong> have a natural (\u2013) negative charge<\/strong> \u00e0 Attract<\/strong> Stain<\/strong>)<\/p>\n\n\n\n\n\n\n\n\n\n\n
<\/td>\n<\/tr>\n
<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n<\/td>\n<\/tr>\n

BASIC stain colors <\/strong>\u00e0<\/td>\n<\/tr>\n
Direct Stain<\/strong><\/td>\nSimple Procedure \u2013 BASIC<\/strong> stains used \/ Stain organism<\/strong> Directly<\/strong><\/td>\n<\/tr>\n
Acidic <\/strong>Stain<\/strong><\/p>\n<\/td>\n<\/td>\n <\/p>\n<\/td>\n(-) Anionic Chromophores<\/p>\n<\/td>\nColored part Negative (-) charge (stain repelled<\/strong> by cells)<\/p>\n<\/td>\n<\/td>\nStain alkaline structures \/ work best in acidic<\/em> low Ph<\/p>\n<\/td>\nBackground (Slide) is stained<\/strong> \/ cells remain CLEAR<\/strong><\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n<\/td>\n<\/tr>\n

\nAcidic stain colors<\/strong>\u00e0<\/strong><\/td>\n<\/tr>\n
Negative<\/strong> Stain<\/strong><\/p>\n<\/td>\n<\/td>\n <\/p>\n<\/td>\nNO heat fixing<\/strong><\/p>\n<\/td>\nAcidic Stains Used<\/strong>:<\/p>\n<\/td>\n<\/td>\n1.        colors background<\/p>\n<\/td>\n2.       Leaves cells clear<\/p>\n<\/td>\n<\/tr>\n
Capsule staining: <\/strong><\/p>\n<\/td>\n<\/td>\n+ Positive stain colors the body<\/p>\n<\/td>\n– Negative stain colors the background<\/p>\n<\/td>\nTreponema pallidum<\/em> \u2013 will not stain directly<\/p>\n<\/td>\n<\/td>\nCausative agent of syphilis<\/p>\n<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

All of the information which is here came from other websites.  The answers are correct as far as I know.  If you find anything that seems incorrect, I would appreciate hearing about it so that I can make corrections. Purpose of Staining:  increases Contrast & Resolution   Provide contrast (easier to see cells against background […]<\/p>\n","protected":false},"author":132804860,"featured_media":533,"parent":73,"menu_order":5,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_coblocks_attr":"","_coblocks_dimensions":"","_coblocks_responsive_height":"","_coblocks_accordion_ie_support":"","footnotes":""},"class_list":["post-532","page","type-page","status-publish","has-post-thumbnail","hentry"],"jetpack_likes_enabled":true,"jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/P9vUsN-8A","jetpack-related-posts":[{"id":535,"url":"https:\/\/my-all-about-you.com\/en\/reference\/microbiology-lab-231\/microbiology-lab-231-study-guides\/","url_meta":{"origin":532,"position":0},"title":"Microbiology Lab Study Guides","author":"myallaboutyou","date":"July 21, 2018","format":false,"excerpt":"\u00a0 \u00a0Study Guide Examination 2 Technique used for pure culture isolation? 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