{"id":109,"date":"2018-01-12T11:29:02","date_gmt":"2018-01-12T17:29:02","guid":{"rendered":"https:\/\/my-all-about-you.com\/?page_id=109"},"modified":"2026-03-20T21:49:51","modified_gmt":"2026-03-21T02:49:51","slug":"microbiology","status":"publish","type":"page","link":"https:\/\/my-all-about-you.com\/en\/reference\/microbiology\/","title":{"rendered":"Microbiology"},"content":{"rendered":"<p>Microbiology is the study of microorganisms. They can be single cellular, multicellular, or acellular. Microbiology incorporates numerous disciplines found within the field of microbiology.<br \/>\nDisciplines are:<br \/>\n\u2022 virology<br \/>\n\u2022 mycology<br \/>\n\u2022 parasitology<br \/>\n\u2022 bacteriology<br \/>\nThe years from 1857-1914 are called the Golden Age of Microbiology. Some of the famous members found within this field are:<img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" data-attachment-id=\"199\" data-permalink=\"https:\/\/my-all-about-you.com\/en\/reference\/microbiology\/famous-microbiologist\/\" data-orig-file=\"https:\/\/i0.wp.com\/my-all-about-you.com\/wp-content\/uploads\/2018\/01\/famous-microbiologist.gif?fit=1122%2C793&amp;ssl=1\" data-orig-size=\"1122,793\" data-comments-opened=\"1\" data-image-meta=\"{&quot;aperture&quot;:&quot;0&quot;,&quot;credit&quot;:&quot;&quot;,&quot;camera&quot;:&quot;&quot;,&quot;caption&quot;:&quot;&quot;,&quot;created_timestamp&quot;:&quot;0&quot;,&quot;copyright&quot;:&quot;&quot;,&quot;focal_length&quot;:&quot;0&quot;,&quot;iso&quot;:&quot;0&quot;,&quot;shutter_speed&quot;:&quot;0&quot;,&quot;title&quot;:&quot;&quot;,&quot;orientation&quot;:&quot;0&quot;}\" data-image-title=\"Famous Microbiologist\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/my-all-about-you.com\/wp-content\/uploads\/2018\/01\/famous-microbiologist.gif?fit=1024%2C724&amp;ssl=1\" class=\"alignnone size-full wp-image-199\" src=\"https:\/\/i0.wp.com\/my-all-about-you.com\/wp-content\/uploads\/2018\/01\/famous-microbiologist.gif?resize=1122%2C793&#038;ssl=1\" alt=\"Famous Microbiologist\" width=\"1122\" height=\"793\" \/><\/p>\n<p>Binomial nomenclature is composed of two italicized words. The first word which is the Genus, is capitalized, and the second word, is the species designation, is in smaller case.<\/p>\n<p>The standard resource for identifying prokaryotes, bacteria, is Bergey&#8217;s Manual of Determinative Bateriology.<\/p>\n<p>To be seen without a microscope, it needs to measure about 100 micrometers.\u00a0 Most microorganisms are smaller than that.\u00a0 Microorganisms differ between each other in size, structure, habitat, metabolism as well as other characteristics.\u00a0 Micros are found in Archaea, Bacteria, and Eukarya life domains.<\/p>\n<p>Microbes in the Bacteria &amp; Archaea domains are all prokayotes (lack nucleus).\u00a0 Cyanobacterium is a prokayotic microorganism.\u00a0 Eukaryotes are Eukaya (has nucleus)Viruses although are microorganisms do not fall within any of the domains of life.\u00a0 Virus is acellular.\u00a0 Peptidoglycan is the ones with cell walls.<\/p>\n<p>Bacteria is a prokaryot because their DNA is not within a true nucleus.\u00a0 It is found everywhere.\u00a0 Yeast is a type of fungal microorganism. \u00a0Alga is photosynthetic.<\/p>\n<p>Otzi te Iceman was discovered frozen in the Alps.\u00a0 Scientists believe that he died of Lyme disease.<\/p>\n<p>Pathogens cause disease.\u00a0 Most are good.\u00a0 They are named for shape.\u00a0 Common shapes are spherical (coccus), rod-shaped (bacillus) or curved (spirillum), spirochete, or vibrio.\u00a0 Bacteria is very versatile.<\/p>\n<p>Archaea unicellular prokaryote.\u00a0 They are different from bacteria in their evolutionary history as well as numerous other differences.\u00a0 Their cell walls are psuedopetidoglycan. None of these have been shown to be a pathogen.<\/p>\n<p><strong>Please refer to your book for addition information.<\/strong><\/p>\n<p><strong>Ebola<\/strong><\/p>\n<p>Clinical Symptoms<\/p>\n<ul>\n<li>Incubation period\u2014Approximately 43 days<\/li>\n<li>Acute illness with rapid onset of fever (104\u2070 &#8211; 109\u2070 F)<\/li>\n<li>Sore throat<\/li>\n<li>Massive vomiting<\/li>\n<li>Diarrhea (3 gallons\/day)<\/li>\n<li>Poor Kidney Function<\/li>\n<li>Poor Liver Function<\/li>\n<li>Internal &amp; External bleeding (bleed out of every body opening)<\/li>\n<li>Lyses or bursting of Red Blood Cells (RBC)<\/li>\n<\/ul>\n<p>Treatment<\/p>\n<p>None<\/p>\n<p>Hydrate Slow vomiting &amp; diarrhea Antibodies boost the immune system.\u00a0 They do not kill viruses<\/p>\n<p>Structure<\/p>\n<ul>\n<li>850 -920 nanometers<\/li>\n<li>Single strand of RNA virus<\/li>\n<li>Non-Segmented<\/li>\n<li>Long thread-like linear molecule<\/li>\n<li>Has an extra protein coat<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.cdc.gov\/vhf\/ebola\/index.html\" target=\"_blank\" rel=\"noopener\">Ebola<\/a> is a member of the filovirus family (Marburg virus).\u00a0 Filovirus can cause severe hemorrhagic fever, which symptoms are, fever, vomiting, prostration, petechial hemorrhages, hypotension, and muscle pain.\u00a0 There are only two members of this virus family (Ebola &amp; <a href=\"https:\/\/www.medicinenet.com\/marburg_virus_history_symptoms_and_treatment\/article.htm#marburg_virus_disease_facts\" target=\"_blank\" rel=\"noopener\">Marburg virus<\/a>).\u00a0 Ebola have five strains with one Ebola-Reston not causing severe disease in humans.<\/p>\n<p>The Filovirus was discovered in 1967 in Germany and Yugoslavia.\u00a0 Researchers were analyzing tissue of a green monkey, which had the disease.\u00a0 Researchers contracted the disease.\u00a0 This virus was named after where the outbreak occurred Marburg, Germany.\u00a0 Ebola was first discovered in 1976 in Africa.\u00a0 There is some evidence that it was originally contracted from bats.\u00a0 Once humans are infected, it spreads from person to person.<\/p>\n<p>Death rate is 23 to 90%.\u00a0 To reduce risk of contracting from another human wear PPE, do not have contact with bodily fluids such as blood, and other secretions.\u00a0 Do not expose yourself to animals out of Africa, which may have the disease.<\/p>\n<p>Currently the medical community is working on a vaccine.\u00a0 Researchers at <a href=\"http:\/\/www.hhmi.org\/bulletin\/fall-2015\/test-tells-tale\" target=\"_blank\" rel=\"noopener\">Howard Hughes Medical Institute<\/a> have developed at blood test (Virscan) which will tell doctors the patient complete viral history.<\/p>\n<p>The information provided above was from my Microbiology class. \u00a0In writing this blog, the CDC and MedicineNet.com were used as references.\u00a0 Please check out the video on <a href=\"https:\/\/www.pbs.org\/wgbh\/frontline\/film\/ebola-outbreak\/\" target=\"_blank\" rel=\"noopener\">PBS <\/a>Front<a href=\"https:\/\/www.pbs.org\/wgbh\/frontline\/film\/ebola-outbreak\/\" target=\"_blank\" rel=\"noopener\">line<\/a> called \u201cEbola\u201d.<\/p>\n<p>Ebola was named after the Ebola River where it was first discovered.\u00a0 There were two major outbreaks in the 90&#8217;s and one in Northwest Africa in 2014.\u00a0 Death occurs within six days.<\/p>\n<p>In the United States there are only 19 negative pressure beds.\u00a0 The closest to Louisiana is Atlanta which has four.<\/p>\n<p>Ebola is an enveloped virus meaning it has an extra protein coat.\u00a0 It is hard for drugs or immunity virus to attach &amp; destroy.<\/p>\n<p>Glycoproteins live on surface of virus.\u00a0 These glycoproteins are made up of carbohydrate chains called glycans.\u00a0 There are two types:<\/p>\n<ol>\n<li>GP1-Attachment (which makes it more potent)<\/li>\n<li>GP2-Fusion of host and viral membranes.<\/li>\n<\/ol>\n<p>Genus Bacillus &#8211; gram positive, aerobic<br \/>\nspore -forming rods<br \/>\nLargely saprophytic &amp; doesn&#8217;t cause<br \/>\nImportance in food preservation<\/p>\n<p>Passion is the genius of genius\u00a0 &#8211; Galileo<br \/>\nDo or not do.\u00a0 There is no try.\u00a0 &#8211; Yoda<\/p>\n<p>Microbes weigh as much as our brain which is 3 lbs. in our body.\u00a0 We have around 65 different microbes in our belly button<\/p>\n<p style=\"text-align:center\">Human Perspective on Micro<\/p>\n<ol>\n<li>Activities of microbe are responsible for all life on earth.\u00a0 We have to have fungus.<\/li>\n<li>Bacteria fix nitrogen so plants can feed breaks down nitrates or nitrites.<\/li>\n<li>Microbes replenish our oxygen.\u00a0 Phytoplankton<\/li>\n<li>Microbes degree organic pollutants &amp; toxic waste.<\/li>\n<li>Food stuff:\u00a0 Cheese, sauerkraut, yogurt, kimchee, bread (yeast), mushrooms, root beer, beer, moonshine, and wine<\/li>\n<li>90% of antibodies are made by microbes.<\/li>\n<li>Genomics &#8211; use fungi for their restriction enzymes<\/li>\n<li>Medical microbe\n<ol>\n<li>old world disease &#8211;plague, TB, yellow fever<\/li>\n<li>emerging world disease &#8211; Ebola, trichomonas, aids, west nile, H1N1 (West Nile)<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<p>Vinods<\/p>\n<p>1\/10 the size of a virus<br \/>\nno outer coat<br \/>\ncause plant disease<br \/>\nshort strands of RNA<\/p>\n<p>Prions (Protein only infectious particle)\u00a0 Short strand Protein<\/p>\n<ol>\n<li>Induce other proteins to become prions<\/li>\n<li>Inheritable<\/li>\n<li>Cause neurogenerative disease<\/li>\n<li>can last for years after death<\/li>\n<li>can penetrate surgical gloves<\/li>\n<\/ol>\n<p>These are all pan resistant which means no antibiotics affect them:<\/p>\n<ol>\n<li>Stenotrophomonas maltophilia enzyme (S. Malt), New Delhi metallo beta lactamase type enzyme (NDM1), <span style=\"display:inline !important;float:none;background-color:transparent;border-collapse:collapse;border-spacing:0 0;color:#444444;font-family:'Segoe UI', Arial, Helvetica, Sans-Serif;font-size:18px;font-style:normal;font-variant:normal;font-weight:400;letter-spacing:normal;list-style-type:none;text-align:left;text-decoration:none;text-indent:0;text-transform:none\">Klebsiella pneumoniae carbapenemase (KPC)<\/span><\/li>\n<\/ol>\n<p>Watch <a href=\"https:\/\/youtu.be\/gE4VzFz9PPo\" target=\"_blank\" rel=\"noopener\">Hunting the Nightmare Bacteria<\/a> on Frontline<\/p>\n<p>Quantum Dot Stain-New<\/p>\n<p>Microscope &amp; staining<\/p>\n<p>Microscopy<\/p>\n<ol>\n<li>light<\/li>\n<li>electron<\/li>\n<li>atomic force<\/li>\n<\/ol>\n<p>Staining<\/p>\n<ol>\n<li>Basic dyes- stains negatively-charged part of cells (nucleic acids, proteins) dyes used:\u00a0 crystal violet, safranin, methylene blue, malachite green<\/li>\n<li>Acidic dyes-stains the background as in a photographic negative, called negative staining, dyes used nigrosine, India ink<\/li>\n<\/ol>\n<p>Mordint<\/p>\n<p>Simple stain<\/p>\n<ol>\n<li>glass slide &amp; place a drop of H2O in the center<\/li>\n<li>Take your inoculating loop &amp; put it in the burner<\/li>\n<li>*Take the heated loop &amp; dip it in your culture.\u00a0 Take the loop put it in H2O droplet &amp; spread all over the slide.\u00a0 This is called a smear.<\/li>\n<li>*Heat &#8220;fix&#8221; the slide over the hot plate<\/li>\n<li>Place a drop of methylene blue or crystal violet on the slide.\u00a0 Let it stand for one minute.\u00a0 Rinse with water, blot dry &amp; observe<\/li>\n<\/ol>\n<p>Gram Stain<\/p>\n<ol>\n<li>Prepare smear &amp; fix<\/li>\n<li>Flood the smear with primary smear-crystal violet let it stand 1 minute &amp; rinse with H2O.<\/li>\n<li>Flood smear with Gram&#8217;s iodine.\u00a0 Let it stand one minute.\u00a0 Rinse with H2O.\u00a0 Iodine acts as a mordant.\u00a0 Mordant increased the likelihood of a structure to take up stain.<\/li>\n<li>Take 95% ethanol and drop by drop rinse the smear.\u00a0 Rinse with H2O.\u00a0 E + OH (ethanol) decolorizes gram negative bacteria.<\/li>\n<li>Flood the smear with your counter stain which is safranin.\u00a0 Let it stand 30 seconds.\u00a0 Rinse with H2O blot dry &amp; observe.\u00a0 Crystal violet will stain gram + purplish to blueish<\/li>\n<\/ol>\n<p>Safranin stain gram negatives pink &#8211; reddish color.<\/p>\n<p class=\"jetpack-slideshow-noscript robots-nocontent\">This slideshow requires JavaScript.<\/p><div id=\"gallery-109-1-slideshow\" class=\"jetpack-slideshow-window jetpack-slideshow jetpack-slideshow-black\" data-trans=\"fade\" data-autostart=\"1\" 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itemscope itemtype=\"https:\/\/schema.org\/ImageGallery\"><\/div>\n<p align=\"center\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\"><span style=\"color:#000000\">Bacterial Morphology<\/span><\/span><\/p>\n<p><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">1.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Spherical or round \u2013 coccus<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">2.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Rod or cylinder \u2013 bacillus<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">3.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Spiral \u2013 spirillum<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">4.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Short, curved rod \u2013 vibrio<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">5.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Star shaped \u2013 prosthecae<\/span><\/span><\/p>\n<p align=\"center\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\"><span style=\"color:#000000\">Some prokaryotic (bacterial structures)<\/span><\/span><\/p>\n<p><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">1.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Ribosome \u2013 make protein<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">2.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Plasmids \u2013 small, circular strand of DNA<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">3.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Nucleoid \u2013 twisted DNA strand<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">4.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Glycocalyx \u2013 layer of polysaccharide lying inside bacterial cell wall<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">5.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Apendayes-<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">a.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Pilus<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">b.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Flagella- consists of a hook for rotation, basal body for anchoring, filament tail for movement<\/span><\/span><\/p>\n<p align=\"center\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\"><span style=\"color:#000000\">Types of flagella<\/span><\/span><\/p>\n<p><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">1.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Monotrichous \u2013 single flagella&#8211;has tail as one end see example\u00a0\u00a0 <\/span><span dir=\"RTL\" lang=\"AR-SA\"><span style=\"font-family:Calibri\">\ufebb <\/span><\/span><\/span><\/p>\n<p><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">2.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Amphitrichous- flagella at both ends\u2014has tail at both ends see example<\/span> <span dir=\"RTL\" lang=\"AR-SA\"><span style=\"font-family:Calibri\">\ufebb<\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:20pt\">~<\/span><\/span><\/p>\n<p><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">3.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Lophotrichous-tuft of flagella\u2014has tufted tails on bottom see example <\/span><span style=\"font-family:Calibri\">\ua65a<\/span><\/span><\/p>\n<p><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">4.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Perithrichous-flagella all over\u2014has tufted tails all over see example <\/span><span style=\"font-family:Calibri\">\ua670<\/span><\/span><\/p>\n<p align=\"center\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\"><span style=\"color:#000000\">Biofilms<\/span><\/span><\/p>\n<p><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\"><span style=\"color:#000000\">Communities or group of microorganisms that attach to the surfaces of animate objects, heart valves, bone tissues, etc and inanimate structures prosthetic in plants, catheters, etc. only effective treatment K removal of infected tissues<\/span><\/span><\/p>\n<p align=\"center\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\"><span style=\"color:#000000\">Bacterial Colonies<\/span><\/span><\/p>\n<p><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\"><span style=\"color:#000000\">Identify by:<\/span><\/span><\/p>\n<p><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">1.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Form shape<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">2.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Elevation- cross section<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">3.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Margin-magnified edge of colony<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">4.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Surface-appearance<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">5.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Opacity-clear, opaque, translucent<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">6.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Chromogenesis-color<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">7.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Consistency<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">8.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Odor<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">9.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Emulsifiability<\/span><\/span><\/p>\n<p><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\"><span style=\"color:#000000\">Rudolph Virchow (1821-1902)<\/span><\/span><\/p>\n<p><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">1.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Father of modern day public health<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">2.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Shaped modern day public health<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">3.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Discovered correlation between cancerous &amp; normal cells, modern day autopsy procedures, hair analysis<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">4.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Names: leukemia, chordoma, embolism, thrombosis, agenesis, parenchyma, osteoid, spina bifida<\/span><\/span><\/p>\n<p><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\"><span style=\"color:#000000\">Anton von Leeuwenhoek<\/span><\/span><\/p>\n<p><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">1.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Father of microbiology<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">2.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Discovered protists &amp; bacteria<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">3.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Distilling H<sub>2<\/sub>O to kill contaminants<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">4.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Described bacterial motility<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">5.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Single-celled (ocular) microscope<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">6.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Most published author in the Royal Society of Great Britain<\/span><\/span><\/p>\n<p><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\"><span style=\"color:#000000\">William Jaffray<\/span><\/span><\/p>\n<p><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">1.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Scotsman<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">2.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Lived near Stirling<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">3.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Interest was in eradication of small pox through variolation &amp; family &amp; neighbors using variolation<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">4.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">After 1796, he inoculated 500 nearby families, 2 families chose no inoculation<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">5.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">President of Royal Society gave him equipment to do much more inoculation.<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">6.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Set up shop in his cottage (1808), travelled throughout Scotland inoculating people.\u00a0 Took neither payment nor a meal.<\/span><\/span><\/p>\n<p align=\"center\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\"><span style=\"color:#000000\">Endospores<\/span><\/span><\/p>\n<p><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">1.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Dormant form of a bacterium that is formed by reorganized the cytoplasm.<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">2.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Spore has great resistance to outside influences heat, radiation, dessication, chemicals<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">3.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Can remain dormant for years\u00a0\u00a0 50-100 years<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">4.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Will germinate if conditions become favorable<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">5.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Size, shape, &amp; position of an endospore is constant within a species &amp; has taxonomic value<\/span><\/span><\/p>\n<p align=\"center\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\"><span style=\"color:#000000\">Types of Endospores<\/span><\/span><\/p>\n<p><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">1.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Central-middle of cell<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">2.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Terminal-end of cell<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">3.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Subterminal-between middle &amp; end<\/span><\/span><\/p>\n<p style=\"text-align:center\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\"><span style=\"color:#000000\">EMB agar (eosin methylene blue)<\/span><\/span><\/p>\n<p><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">1.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Selective &amp; differential<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">2.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Used to isolate gram (-)<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">3.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Methylene dye in small amounts<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">4.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">Eosin dye responds to changes in pH \u2013 colorless to black under acidic conditions<br \/>\n<\/span><\/span><span style=\"color:#000000\"><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">5.<span style=\"font:7pt 'Times New Roman';margin:0\">\u00a0\u00a0\u00a0\u00a0 <\/span><\/span><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\">EMB contains lactose &amp; sucrose, but no glucose for energy<\/span><\/span><\/p>\n<p><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\"><span style=\"color:#000000\">Lactose fermenting bacteria (usually enteric) acidify EMB &amp; colonies appear black with green sheen (E. coli, Klebsiella, Enterobacter)<\/span><\/span><\/p>\n<p><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\"><span style=\"color:#000000\">Lactose non-fermenters are translucent to pink (salmonella, shigella, and pseudomonas<\/span><\/span><\/p>\n<p><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\"><span style=\"color:#000000\">The EMB selects for growth of gram (-) bacteria &amp; differentiates between genera of gram (-)<\/span><\/span><\/p>\n<p><span style=\"margin:0;line-height:115%;font-family:'Georgia', 'serif';font-size:14pt\"><span style=\"color:#000000\">We must be willing to get rid of the life we have planned so as to have the life that is waiting for us.\u00a0 Joseph Campbell<\/span><\/span><\/p>\n<p><span style=\"color:#000000\">Dynamics of Prokaryotic (Bacterial) Growth<\/span><\/p>\n<p><span style=\"color:#000000\">Only 1% of all prokaryotes can be cultured as a pure culture.\u00a0 You need a good growth medium to grow which is agar.\u00a0 It was developed by Robert Koch.\u00a0 Agar is:<\/span><\/p>\n<ol>\n<li><span style=\"color:#000000\">specific nutrient solution<\/span><\/li>\n<li><span style=\"color:#000000\">degraded by bacteria<\/span><\/li>\n<li><span style=\"color:#000000\">translucent<\/span><\/li>\n<li><span style=\"color:#000000\">destroyed at high temperatures<\/span><\/li>\n<\/ol>\n<p><span style=\"color:#000000\">Environmental factors that influence microbial growth are temperature, oxygen, pH, water.<\/span><\/p>\n<p><span style=\"color:#000000\">Temperatures:\u00a0 psychrophiles, phychrotrophs, and mesophiles<\/span><br \/>\n<span style=\"color:#000000\">Oxygen:\u00a0 obligate aerobes obligate anaerobes facultative anaerobes microaerophiles, aero tolement anaerobes<\/span><br \/>\n<span style=\"color:#000000\">pH:\u00a0 neutrophiles, acidophiles, alkalophiles<\/span><br \/>\n<span style=\"color:#000000\">Water:\u00a0 halophiles-love salty water<\/span><\/p>\n<p><span style=\"color:#000000\">Cultivating Prokaryotes<\/span><\/p>\n<ol>\n<li><span style=\"color:#000000\">complex media<\/span><\/li>\n<li><span style=\"color:#000000\">chemically defined media<\/span><\/li>\n<li><span style=\"color:#000000\">selective media- adds ingredients that inhibit the growth of orgs other than the one bring sought<\/span><\/li>\n<li><span style=\"color:#000000\">differential-contains an ingredient that can be changed by bacteria in a recognizable way.<\/span><\/li>\n<\/ol>\n<p><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" data-attachment-id=\"493\" data-permalink=\"https:\/\/my-all-about-you.com\/en\/reference\/microbiology\/hemolysis\/\" data-orig-file=\"https:\/\/i0.wp.com\/my-all-about-you.com\/wp-content\/uploads\/2018\/02\/hemolysis.jpg?fit=826%2C537&amp;ssl=1\" data-orig-size=\"826,537\" data-comments-opened=\"1\" data-image-meta=\"{&quot;aperture&quot;:&quot;0&quot;,&quot;credit&quot;:&quot;&quot;,&quot;camera&quot;:&quot;&quot;,&quot;caption&quot;:&quot;&quot;,&quot;created_timestamp&quot;:&quot;0&quot;,&quot;copyright&quot;:&quot;&quot;,&quot;focal_length&quot;:&quot;0&quot;,&quot;iso&quot;:&quot;0&quot;,&quot;shutter_speed&quot;:&quot;0&quot;,&quot;title&quot;:&quot;&quot;,&quot;orientation&quot;:&quot;0&quot;}\" data-image-title=\"hemolysis\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/my-all-about-you.com\/wp-content\/uploads\/2018\/02\/hemolysis.jpg?fit=826%2C537&amp;ssl=1\" class=\"alignnone size-full wp-image-493\" src=\"https:\/\/i0.wp.com\/my-all-about-you.com\/wp-content\/uploads\/2018\/02\/hemolysis.jpg?resize=826%2C537&#038;ssl=1\" alt=\"hemolysis\" width=\"826\" height=\"537\" \/><\/p>\n<p>The zone hemolysis is the area where blood is breaking down.\u00a0 The above are examples.\u00a0 Please refer to professor notes for the example he\/she gives.<\/p>\n<p>Detecting and Meaning<\/p>\n<p style=\"padding-left:30px\">Bacterial Growth<\/p>\n<ol>\n<li>direct cell count<\/li>\n<li>direct microscopic count\n<ol>\n<li>coulter counter<\/li>\n<li>flow cytometer<\/li>\n<\/ol>\n<\/li>\n<li>viable (alive) cell count\n<ol>\n<li>most probable #<\/li>\n<li>plate count\n<ol>\n<li>pour plate<\/li>\n<li>spread plate<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<\/li>\n<li>membrane filtration<\/li>\n<\/ol>\n<p>Growth curve<\/p>\n<p>Bacterial Growth<\/p>\n<ol>\n<li>direct cell count<\/li>\n<li>direct microscopic count\n<ol>\n<li>coulter counter<\/li>\n<li>flow cytometer<\/li>\n<\/ol>\n<\/li>\n<li>viable (alive) cell count\n<ol>\n<li>most probable #<\/li>\n<li>plate count\n<ol>\n<li>pour plate<\/li>\n<li>spread plate<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<\/li>\n<li>membrane filtration<\/li>\n<\/ol>\n<p>Growth curve<\/p>\n<p>5 stages<\/p>\n<ol>\n<li>lag-extensive macromolecule &amp; ATP synthesis<\/li>\n<li>Exponential or log &#8211; cells multiply exponentially\n<ol>\n<li>primary metabolite &#8211; used as flavoring agents &amp; food supplements<\/li>\n<li>secondary metabolites produce antibiotics<\/li>\n<\/ol>\n<\/li>\n<li>stationary phase-#of viable (living) cells stay constant<\/li>\n<li>death-#of viable cells decrease exponentially<\/li>\n<li>phase of prolonged decline-gradual decrease in # of viable cells overtime (days, weeks, years, and decades)<\/li>\n<\/ol>\n<p>Microbial growth<\/p>\n<p>controls-<\/p>\n<ol>\n<li>physical &#8211; heat, wash, filter, irradiate<\/li>\n<li>chemical-antimicrobial chems<\/li>\n<\/ol>\n<p>sterilization &#8211; removing of or destroying all microbes on or in a product.\u00a0 Disinfection-eliminating most or all disease<\/p>\n<p>Situational Considerations<\/p>\n<ol>\n<li>daily life<\/li>\n<li>hospitals<\/li>\n<li>labs<\/li>\n<li>food &amp; food production<\/li>\n<\/ol>\n<p>Using heat to destroy<\/p>\n<ol>\n<li>moist heat<\/li>\n<li>dry heat<\/li>\n<li>pasteurization<br \/>\nWhy is heat so good?\u00a0 It is cheap quick<\/li>\n<\/ol>\n<p>Chemicals<\/p>\n<ol>\n<li>sterilants<\/li>\n<li>disinfectants<\/li>\n<\/ol>\n<p>Potential risk of infection<\/p>\n<ol>\n<li>critical items &#8211; scalpel, gloves, needles<\/li>\n<li>semi-critical &#8211; endoscope, trachea tube<\/li>\n<li>non -critical &#8211;\u00a0 bp cuff<\/li>\n<\/ol>\n<p>Selecting the appropriate germicide<\/p>\n<ol>\n<li>toxicity<\/li>\n<li>activity in the presence of organic matter<\/li>\n<li>compatibility<\/li>\n<li>residue<\/li>\n<li>cost<\/li>\n<li>storage &amp; stability<\/li>\n<li>environment risk<\/li>\n<\/ol>\n<p>Preservation techniques<\/p>\n<ol>\n<li>refrigerate, freeze, lypophilize &#8211; freeze dry<\/li>\n<li>salts<\/li>\n<li>sugars<\/li>\n<\/ol>\n<p>Classes of Germicides<\/p>\n<ol>\n<li>alcohol-antiseptic\u00a0 kill bacteria &amp; fungi<\/li>\n<li>aldehydes &#8211; formaldehyde, inactive proteins &amp; nuclei acids<\/li>\n<li>br guanides &#8211; antiseptic, chlorhexide kills bacteria &amp; fungi<\/li>\n<li>ethylene oxide &#8211; gas, kills microbes &amp; endospores<\/li>\n<li>halogens, CHI disinfect drinking waste water<\/li>\n<li>ozone- same as halogens<\/li>\n<li>metals\u00a0 silver, inhibits protein &amp; enzyme function \u00a0 Copper is the best of these<\/li>\n<li>peroxygen H2O2 &#8211; sterilize juice &amp; milk juice containers<\/li>\n<li>Pheholic compounds triclosan &amp; hexachlorophene\u00a0 &#8211; denatures proteins<\/li>\n<li>Quats &#8211; food prep surfaces<\/li>\n<\/ol>\n<p>Human Microbiomes<\/p>\n<p>Disinfection in clinical and non-clinical setting ways to disinfect non-porous surface<\/p>\n<ol>\n<li>steam<\/li>\n<li>mists<\/li>\n<li>foams<\/li>\n<li>UV<\/li>\n<li>liquid disinfectants<\/li>\n<li>microbiocide surfaces<\/li>\n<\/ol>\n<p>Chemical disinfection<\/p>\n<p>Most are ineffective and harmful.\u00a0 Why?\u00a0 Very, very long contact time}hospital centered or a hospital setting<\/p>\n<p>Gases, Mists, Foams<\/p>\n<p>sit for 2-24 hours, seal the room and be unoccupied<\/p>\n<p>Ozone <span style=\"margin:0;line-height:115%;font-family:'Calibri', 'sans-serif';font-size:12pt\"><span style=\"color:#000000\">O<sub>3<\/sub><\/span><\/span><\/p>\n<p>Disinfect rooms, use it as a gas, Dangerous- lead to free radical formation in cells.<\/p>\n<p style=\"text-align:center\"><strong>Cancer<\/strong><\/p>\n<p>Questions they must ask if a tumor appears cancerous<\/p>\n<ol>\n<li>Size of primary tumor<\/li>\n<li>Has it invaded surrounding tissue<\/li>\n<li>Has it metastasized (spread)<\/li>\n<li>Has it invaded any regional lymph nodes<\/li>\n<\/ol>\n<p>TNM System for Identification<\/p>\n<p>T = Tumor<\/p>\n<p><span style=\"margin:0;color:#3d596d;line-height:115%;font-family:'Georgia', 'serif';font-size:13.5pt\">T<sub>0<\/sub><\/span> = no tumor;<br \/>\n<span style=\"margin:0;color:#3d596d;line-height:115%;font-family:'Georgia', 'serif';font-size:13.5pt\">T<sub>1 <\/sub>\u00a0= very small;<br \/>\n<\/span><span style=\"margin:0;color:#3d596d;line-height:115%;font-family:'Georgia', 'serif';font-size:13.5pt\">T<sub>2 <\/sub>\u00a0= small;<br \/>\n<\/span><span style=\"margin:0;color:#3d596d;line-height:115%;font-family:'Georgia', 'serif';font-size:13.5pt\">T<sub>3 <\/sub>\u00a0= medium;<br \/>\n<\/span><span style=\"margin:0;color:#3d596d;line-height:115%;font-family:'Georgia', 'serif';font-size:13.5pt\">T<sub>4 <\/sub>\u00a0= large<\/span><\/p>\n<p>N = Lymph node<\/p>\n<p><span style=\"margin:0;color:#3d596d;line-height:115%;font-family:'Georgia', 'serif';font-size:13.5pt\">N<sub>0 <\/sub>\u00a0= no nodes involved<\/span>;<br \/>\n<span style=\"margin:0;color:#3d596d;line-height:115%;font-family:'Georgia', 'serif';font-size:13.5pt\">N<sub>1 <\/sub>\u00a0= 1 node involved less than 3 cm;<br \/>\n<\/span><span style=\"margin:0;color:#3d596d;line-height:115%;font-family:'Georgia', 'serif';font-size:13.5pt\">N<sub>2 <\/sub>\u00a0= medium node involved \u00a03-6 cm &amp; multiple small nodes;<br \/>\n<\/span><span style=\"margin:0;color:#3d596d;line-height:115%;font-family:'Georgia', 'serif';font-size:13.5pt\">N<sub>3 <\/sub>\u00a0= single node larger than 6 cm<\/span><\/p>\n<p>M = Metastasized<\/p>\n<p><span style=\"margin:0;color:#3d596d;line-height:115%;font-family:'Georgia', 'serif';font-size:13.5pt\">M<sub>0 <\/sub>\u00a0= no spread<\/span>;<br \/>\n<span style=\"margin:0;color:#3d596d;line-height:115%;font-family:'Georgia', 'serif';font-size:13.5pt\">M<sub>1 <\/sub>\u00a0= spread<\/span><\/p>\n<p>Diagnosis = clinical symptoms<br \/>\nPrognosis = probable outcome<\/p>\n<p>Cancer treatments<\/p>\n<ol>\n<li>Surgery<\/li>\n<li>Chemo<\/li>\n<li>Radiation<\/li>\n<\/ol>\n<p>Cancer Warning Signs<\/p>\n<ol>\n<li>lump or thickening in the breast or elsewhere<\/li>\n<li>nagging cough or hoarseness<\/li>\n<li>changing appearance of mole or wart<\/li>\n<li>indigestion or difficulty in swallowing<\/li>\n<li>change in bladder or bowel<\/li>\n<li>unusual bleeding or discharge<\/li>\n<li>sore that doesn&#8217;t heal<\/li>\n<\/ol>\n<p>Staph &#8211; becoming XDR<\/p>\n<p><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" data-attachment-id=\"523\" data-permalink=\"https:\/\/my-all-about-you.com\/en\/reference\/microbiology\/staph\/\" data-orig-file=\"https:\/\/i0.wp.com\/my-all-about-you.com\/wp-content\/uploads\/2018\/04\/staph.jpg?fit=450%2C158&amp;ssl=1\" data-orig-size=\"450,158\" data-comments-opened=\"1\" data-image-meta=\"{&quot;aperture&quot;:&quot;0&quot;,&quot;credit&quot;:&quot;&quot;,&quot;camera&quot;:&quot;&quot;,&quot;caption&quot;:&quot;&quot;,&quot;created_timestamp&quot;:&quot;0&quot;,&quot;copyright&quot;:&quot;&quot;,&quot;focal_length&quot;:&quot;0&quot;,&quot;iso&quot;:&quot;0&quot;,&quot;shutter_speed&quot;:&quot;0&quot;,&quot;title&quot;:&quot;&quot;,&quot;orientation&quot;:&quot;0&quot;}\" data-image-title=\"staph\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/my-all-about-you.com\/wp-content\/uploads\/2018\/04\/staph.jpg?fit=450%2C158&amp;ssl=1\" class=\"alignnone size-full wp-image-523\" src=\"https:\/\/i0.wp.com\/my-all-about-you.com\/wp-content\/uploads\/2018\/04\/staph.jpg?resize=450%2C158&#038;ssl=1\" alt=\"staph\" width=\"450\" height=\"158\" \/><\/p>\n<p>Pyogenic &#8211; pus producer<\/p>\n<p style=\"padding-left:30px\">Staphylococcus aureus<\/p>\n<ol>\n<li>Gram positive<\/li>\n<li>Spherical<\/li>\n<li>Non-Motile<\/li>\n<li>Produces no spores<\/li>\n<li>Colonies are round, raised, golden yellow<\/li>\n<\/ol>\n<p>Toxin and Enzymes<\/p>\n<ol>\n<li>Staph produces an enzyme called betalactamase \u2013 makes it resistant to penicillins and cephalosporins<\/li>\n<li>Cell wall structure is unique and resistant to methicillin (MRSA \u2013 methicillin resistant Staph aureus)<\/li>\n<\/ol>\n<p>Side note: CAMRSA &#8211; Communicative associative methicillin resistant staph aureus<\/p>\n<ol start=\"3\">\n<li>Plasmids (round circular piece of DNA) \u2013 carry genes resistance tetracyclines, amino glycosides, and erythromycins<\/li>\n<li>Exhibits \u201ctolerance\u201d. Inhibits but not killed<\/li>\n<li>Exotoxin \u2013 skin necrosis (rotting)<\/li>\n<li>Exotoxin C \u2013 fever producing (106\u00b0 F)<\/li>\n<li>Hemolysins \u2013 cause red blood cells to burst<\/li>\n<li>Leukocidins \u2013 kills white blood cells<\/li>\n<li>Enterotoxin \u2013 food poisoning<\/li>\n<li>Coagulase \u2013 causes plasma to clot<\/li>\n<li>Hyaluronidase \u2013 spreading factor<\/li>\n<li>Exfoliative toxin \u2013 causes \u201cscalded skin\u201d syndrome<\/li>\n<li>Postulated toxin \u2013 causes TSS \u2013 toxic shock syndrome<\/li>\n<\/ol>\n<p>Other manifestations:<\/p>\n<ol>\n<li>Empyema \u2013 accumulation of pus in a body cavity<\/li>\n<li>Severe acne<\/li>\n<li>Impetigo \u2013 small pustules that ooze a yellowish mustard like liquid<\/li>\n<li>Pneumonia (pus in the lung)<\/li>\n<li>Meningitis<\/li>\n<li>Endocarditis<\/li>\n<\/ol>\n<p>Treatment Options<\/p>\n<ol>\n<li>No vaccines<\/li>\n<li>Drain lesion<\/li>\n<li>Best medications \u2013 vancomycin, clindamycin, rifampin, mupirocin (cream that can be used in the nasal cavity)<\/li>\n<\/ol>\n<p>Vaccines \u2013 substances that are found in vaccines:\u00a0 (1.2 \u2013 2B people saved from vaccinations)<\/p>\n<ol>\n<li>Aluminum \u2013 act as an adjuvant (enhances) to promote a more potent response and a more persistent response.<\/li>\n<li>Antibiotics \u2013 prevents the growth of germs during production and storage of the vaccine.<\/li>\n<li>Egg protein \u2013 used in production of influenza and yellow fever drugs.<\/li>\n<li>Formaldehyde \u2013 inactivates bacterial products for toxoid vaccines, kills unwanted viruses and bacteria that might contaminate the vaccine during production. It is removed from the vaccine before packaging.<\/li>\n<li>MSG \u2013 stabilizer for the vaccine in the presence of heat, light, acidity or humidity.<\/li>\n<li>*Thimerosol \u2013 mercury containing preservative that prevents growth of harmful bacteria.<\/li>\n<\/ol>\n<p>Herd immunity \u2013 protecting a whole community from disease by immunizing a critical mass of its populace.<\/p>\n<p>Vaccine protects not only the one person, but breaks the chain of an infection\u2019s transmission, it also protects non-immunized individuals.\u00a0 To do this a certain % of the populace must be vaccinated.<\/p>\n<p>Ways to become immune to a disease<\/p>\n<ol>\n<li>Be exposed to a pathogen<\/li>\n<li>Vaccinate<\/li>\n<\/ol>\n<p>Infectious features of a microbe.<\/p>\n<ol>\n<li>Transmissibility<\/li>\n<li>Severity<\/li>\n<\/ol>\n<p>These affect a disease\u2019s threshold (minimum % of an immune individuals and community needs to prevent an outbreak<\/p>\n<p>To set a threshold one uses a value \u2013 basic reproductive # or RO<\/p>\n<p>The RO represents how many people in an unprotected population, one infected person can pass the disease along to<\/p>\n<p>RO for measles is 12-18<\/p>\n<p>Polio 5-7<\/p>\n<p>Higher the %, higher the immunity threshold must be to protect the community<\/p>\n<p>Measles 95%<\/p>\n<p>Polio 80%<\/p>\n<p>Factors involved in calculating a specific threshold:<\/p>\n<ol>\n<li>How effective the vaccine for a given disease is?<\/li>\n<li>How long lasting the immunity is from vaccination and infection?<\/li>\n<li>Which populations form critical links in transmission of the disease (very young, very old, immune compromised, religion groups)<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<table>\n<tbody>\n<tr>\n<td width=\"102\">Disease<\/td>\n<td width=\"102\">RO<\/td>\n<td width=\"108\">Threshold %<\/td>\n<\/tr>\n<tr>\n<td width=\"102\">Mumps<\/td>\n<td width=\"102\">4-7<\/td>\n<td width=\"108\">75-86<\/td>\n<\/tr>\n<tr>\n<td width=\"102\">Polio<\/td>\n<td width=\"102\">5-7<\/td>\n<td width=\"108\">80-86<\/td>\n<\/tr>\n<tr>\n<td width=\"102\">Smallpox<\/td>\n<td width=\"102\">5-7<\/td>\n<td width=\"108\">80-85<\/td>\n<\/tr>\n<tr>\n<td width=\"102\">Diphtheria<\/td>\n<td width=\"102\">6-7<\/td>\n<td width=\"108\">85<\/td>\n<\/tr>\n<tr>\n<td width=\"102\">Rubella<\/td>\n<td width=\"102\">6-7<\/td>\n<td width=\"108\">83-85<\/td>\n<\/tr>\n<tr>\n<td width=\"102\">Pertussis<\/td>\n<td width=\"102\">12-17<\/td>\n<td width=\"108\">92-94<\/td>\n<\/tr>\n<tr>\n<td width=\"102\">Measles<\/td>\n<td width=\"102\">12-18<\/td>\n<td width=\"108\">83-94<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>Koch and germ theory of disease<\/p>\n<p>Robert Koch (1843-1910), physician, studied anthrax early on in his work.\u00a0 It is a disease of cattle, sometimes people.\u00a0 Caused by Bacillus anthracis \u2013 endospore former.\u00a0 Koch established that anthrax bacteria were ever present in the blood of an animal with anthrax disease.\u00a0 He demonstrated that one could take a small amount of blood from a diseased mouse and inject it into a 2<sup>nd<\/sup> disease-free mouse.\u00a0 This mouse then contracted anthrax and died.\u00a0 He took blood from the dead mouse, injected it into another mouse and it got the disease.\u00a0 Furthermore, he found that one could cultivate the bacteria in nutrient-rich fluid outside of an animal body and that after many transfers in other cultures, the bacteria was still highly pathogenic.\u00a0 From these experiments he formulated his famous Koch\u2019s Postulates.<\/p>\n<p>Koch\u2019s Postulates<\/p>\n<ol>\n<li>The organism should be constantly present in animals suffering from the disease and should not be present in healthy individual.<\/li>\n<li>The organism must be cultivated in a pure culture away from the animal body.<\/li>\n<li>Such a culture, when inoculated into susceptible animals, should initiate the characteristic disease symptoms.<\/li>\n<li>The organism should be reisolated from these experimental animals and cultured again in the lab after which it should be the same as the original organisms.<\/li>\n<\/ol>\n<p>Summary of the postulates: specific organisms cause specific diseases.<\/p>\n<p><strong><u>Stenotrophomonas maltiphilia (aerobe)<\/u><\/strong><\/p>\n<p>Multi-drug resistant, Gram (-) bacillus, opportunistic pathogen particularly in hospitalized patients.<\/p>\n<p>It has an inherent ability to adhere to foreign materials and form a biofilm, thus, it is protected from host antimicrobial agents.<\/p>\n<p>It has intrinsic or acquired resistance mechanisms to: betalactamases, penicillinase, cephalo-sporinase, aminoglycoside acetyl transferase to confer resistance.<\/p>\n<p>Epidemiology<\/p>\n<p>High morbidity (clinical symptoms \u2013 fever, etc)<\/p>\n<p>High mortality (21-69%)<\/p>\n<p>Risk factors for acquiring an S-malt infection: ICU, vented, HIV, cancer, CF, catheters, surgery, broad-spectrum antibiotic treatment.<\/p>\n<p>Need strict hand hygiene and contact isolation.<\/p>\n<p>Clinical manifestations \u2013 pneumonia, bacteremia, endocarditis, mastoiditis, peritonitis, UTIs, soft wound infection, ocular infection, meningitis.<\/p>\n<p>Treatment \u2013 hard to discern from other infections.\u00a0 Need much clinical data. Only somewhat effective treatment is:<\/p>\n<p>TMP-SMX +\/or<\/p>\n<p>TCA +\/or ciproflaxin<\/p>\n<p>14 day treatment span<\/p>\n<p>About 41% effective<\/p>\n<p><strong><u>Diversity of Prokaryotes<\/u><\/strong><\/p>\n<p>Organohalide respiring bacteria \u2013 use <u>chlorinated molecules<\/u><\/p>\n<p>Methanogens \u2013 oxidize <u>hydrogen gas<\/u>, make <u>methane<\/u><\/p>\n<p>Purple sulfur and green sulfur bacteria \u2013 use <u>sulfur compounds,<\/u> grow in large masses in sulfur springs.<\/p>\n<p>Lactic acid bacteria \u2013 make <u>lactic acid<\/u>.\u00a0 Can cause disease and make dairy products (yogurt) \u2013 Lacto bacillus<\/p>\n<p>Streptococcus pyogenes \u2013 cause disease \u2013 wound infections, strep throat, rheumatic fever, glomerular nephritis, flesh-eating, TSS<\/p>\n<p>Nitrifers \u2013 oxidize <u>NH3<\/u> and <u>nitrate<\/u>, breakdown<u> N<\/u><\/p>\n<p>Bioluminescent bacteria \u2013 utilize <u>luminol<\/u>, glow in the dark, symbiosis with deep sea fish (angler fish, shrimp) to help attract a mate and score off predators, attract prey.<\/p>\n<p>Prochlorococcus \u2013 smallest known microscopic phytoplankton, marine, numbers are trillion-trillion, responsible for \u00bd of all global photosynthesis <u>(CO2)<\/u><\/p>\n<p>Ice nucleators (IN) \u2013 microbes such as <em>Pseudomonas syringae<\/em> that are found in the upper atmosphere.\u00a0 They influence weather.\u00a0 Dust and soot particles act as seed nuclei for the bacteria to attach to them.\u00a0 Once this happens, the bacteria catalyze ice formation which can then fall to Earth\u2019s surface as rain, sleet or snow.<\/p>\n<p>Snottites \u2013 biofilm of chemotrophic bacteria that oxidize <u>hydrogen sulfide<\/u>.\u00a0 They are found in caves and produce stalagmite and stalgtite-like columns.\u00a0 Also etch vertical and horizontal lines in cave walls.<\/p>\n<p><strong><u>Pathogenicity<\/u><\/strong><\/p>\n<p>Parasite \u2013 one that lives at expense of host<\/p>\n<ol>\n<li>Ectoparasite \u2013 lives on surface of host<\/li>\n<li>Endoparasite \u2013 lives within host<\/li>\n<\/ol>\n<p>Pathogen \u2013 any parasitic organism that produces an infectious disease (ex \u2013 cold virus, hook worm)<\/p>\n<p>Pathogenicity \u2013 ability of a parasitic organism to cause disease<\/p>\n<p>Final outcome of a host\/parasite relationship depends upon:<\/p>\n<ol>\n<li>Virulence of pathogen present \u2013 degree or intensity of pathogenicity.<\/li>\n<li>Number of pathogens present<\/li>\n<li>Host degree of resistance<\/li>\n<\/ol>\n<p>Virulence depends on:<\/p>\n<ol>\n<li>Invasiveness<\/li>\n<li>Infectivity ability to establish a focal point of infection<\/li>\n<li>Pathogenic potential \u2013 degree to which the pathogen can cause morbid symptoms<\/li>\n<\/ol>\n<p>Transport of bacterial pathogen:<\/p>\n<ol>\n<li>Direct contact \u2013 cough, sneeze, body contact<\/li>\n<li>Indirect contact \u2013 soil, H20, food, vectors \u2013 insects, fomites \u2013 inanimate objects<\/li>\n<\/ol>\n<p><u>Studies of the Human Microbiome (full set of microbial genomes that exist in an organism)<\/u><\/p>\n<p>Different body habitats are very different from each other in their microbial communities (mouth, earwax, hair, underarm, toe)<\/p>\n<p>Most diverse \u2013 mouth, nose, 231 phyla<\/p>\n<p>Least diverse \u2013 stomach, toes, 65 phyla<\/p>\n<p>Large differences in stool and intestinal mucosa \u2013 343 genera with the largest genus being Bacteroides.<\/p>\n<p>Fecal bacterium \u2013 anti-inflammatory correlated with pulse and bp<\/p>\n<p>4 largest genera of human microbiomes: Bacteroides, Firmicutes, Actinobacteria, Fecal bacterium<\/p>\n<p>Newborns acquire mother\u2019s vaginal microbiome in natural childbirth, but very little if C-section.\u00a0 Breast fed have a far more diverse microbiota than formula fed (virtually none).<\/p>\n<p>Identical twins have quite dissimilar microbial communities.<\/p>\n<p><u>Gut Ecology and c. diff<\/u><\/p>\n<p>AAD \u2013 antibiotic associated diarrhea \u2013 1<sup>st<\/sup> thought to be associated with Staph, but it is due to killing off healthy gut microbes and leaving a very nasty pathogen present \u2013 clostridium difficile (bloating, gas, diarrhea, pain lasting for months).<\/p>\n<p>Hard to get good microbes in high numbers to defeat c. diff.\u00a0 Best way \u2013 fecal bacteriotherapy \u2013 use host feces (relative), screen it for disease and liquefy, use a feeding tube to ingest.\u00a0 2-3 days back to normal.<\/p>\n<p>How does our microbiota defend against c. diff?<\/p>\n<ol>\n<li>Impair it directly (attack)<\/li>\n<li>Shape the host immune response<\/li>\n<li>Antimicrobial peptide \u2013 Reg III gamma<\/li>\n<li>IgA antibody<\/li>\n<li>Short-chain fatty acids<\/li>\n<li>Occupy space in gut<\/li>\n<li>Colonization defense<\/li>\n<li>Filamentous bacteria stimulate lymphocytes<\/li>\n<\/ol>\n<p style=\"text-align:center\">Salmonella<\/p>\n<ol>\n<li><span style=\"margin:0;color:#3d596d;line-height:115%;font-family:'Georgia', 'serif';font-size:13.5pt\">Use as a <span style=\"text-decoration:underline\">possible treatment<\/span> in <span style=\"text-decoration:underline\">prostrate cancer<\/span>.\u00a0 1.1 million cases worldwide, mostly in developing countries.\u00a0 It is the 4th most common in both sexes, 2nd in men, 5th leading cause of cancer death.<br \/>\n<\/span><\/li>\n<li>Researchers at Swansea University in Wales<\/li>\n<li>They modified the salmonella to render it harmless\u00a0 and engineered cells to use them as a therapeutic molecule to target cancer cells.\u00a0 Coupled with Salmonella the molecule delivers the &#8220;switching off&#8221; gene mechanism to genes in the cancer cells, thus, tumor growth is disrupted.\u00a0 Also attached to the gene mechanism is an anti-cancer drug which is non-toxic to normal body cells &amp; only a single dose is needed to achieve positive results.<\/li>\n<\/ol>\n<p><span style=\"margin:0;color:#3d596d;line-height:115%;font-family:'Georgia', 'serif';font-size:13.5pt\">Phase I<\/span><span style=\"margin:0;color:#3d596d;line-height:115%;font-family:'Georgia', 'serif';font-size:13.5pt\"><br \/>\n<\/span><\/p>\n<p>Prostrate cancer cells have shrunk in the lab when exposed to the modified bacterium<\/p>\n<p>Phase II<\/p>\n<p>Will be used in clinical application trials.<\/p>\n<p>Helminthes (parasitic worms)<\/p>\n<ol>\n<li>Nematodes &#8211; roundworm, pinworm, hookworm<\/li>\n<li>Cestodes &#8211; tapeworms<\/li>\n<li>Trematodes &#8211; flukes<\/li>\n<li>Filavial worms<\/li>\n<\/ol>\n<p>Nematodes-In Phylum Aschelminthes, most common intestinal helminth in man.\u00a0 Diagnose by detection of eggs or larvae in feces, transmission is fecal &#8211; oral route (food, water) or skin penetration by larvae (hookworm).\u00a0 Cylindviral body<\/p>\n<p>Cestodes-Flat, ribbon-shaped body, segmented.\u00a0 Head or scolex has suckers and hooks for attachment.\u00a0 No GI tract instead absorb nutrients.\u00a0 Get from undercooked or raw beef, lamb, pork, or fish.\u00a0 Dipylidium caninum (dog tapeworm)-get from playing with a dog.\u00a0 It is the most common.<\/p>\n<p>Trematodes-Phylum Platyhelminthes, bilaterally symmetrical, flat, leaf-shaped, suckers for attachment and sucking.\u00a0 Most are hermaphrodites (box sex organs in same worm).\u00a0 Larval stage within the egg, larvae can escape into environment when they can find an intermediate host, then get released as a tail-bearing larvae-cercaria.\u00a0 Cercaria can penetrate skin, go to the liver and intestines, mature and lay eggs.<\/p>\n<p>Filaviae (dog &amp; cat heart worm)-slender, thread-like nematodes.\u00a0 Transmitted by bite an arthropod vector (fly, mosquito).\u00a0 Live in lymphatic system or deep connective tissue of host.\u00a0 Produce larvae known as microfilaviae.<\/p>\n<p>Wucheria bancrofti &amp; Brugia Malayi &#8211; cause lymphatic filiariasis-lymphatic channels become blocked &amp; lymphedema results.<\/p>\n<p>Loa loa &#8211; eye worm disease (loiasis)-inhabits deep tissues and can be seen crossing conjunctiva of eye, intense itching, &#8220;creeping feeling&#8221;.<\/p>\n<p>Onchocerca volvulus-river blindness, major public health problem, worms become encapsulated in fibrous nodules &amp; ocular lesions cause blindness.<\/p>\n<p>Drancunculus medinensis (Guinea worm)-long, slender, soft-bodied worms acquired by drinking contaminated water, exude from any body part with an intense burning.<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Microbiology is the study of microorganisms. They can be single cellular, multicellular, or acellular. Microbiology incorporates numerous disciplines found within the field of microbiology. Disciplines are: \u2022 virology \u2022 mycology \u2022 parasitology \u2022 bacteriology The years from 1857-1914 are called the Golden Age of Microbiology. Some of the famous members found within this field are: [&hellip;]<\/p>\n","protected":false},"author":132804860,"featured_media":199,"parent":73,"menu_order":3,"comment_status":"open","ping_status":"open","template":"","meta":{"_coblocks_attr":"","_coblocks_dimensions":"","_coblocks_responsive_height":"","_coblocks_accordion_ie_support":"","footnotes":""},"class_list":["post-109","page","type-page","status-publish","has-post-thumbnail","hentry"],"jetpack_likes_enabled":true,"jetpack_sharing_enabled":false,"jetpack_shortlink":"https:\/\/wp.me\/P9vUsN-1L","jetpack-related-posts":[{"id":532,"url":"https:\/\/my-all-about-you.com\/en\/reference\/microbiology-lab-231\/","url_meta":{"origin":109,"position":0},"title":"Microbiology Lab","author":"myallaboutyou","date":"July 21, 2018","format":false,"excerpt":"All of the information which is here came from other websites.\u00a0 The answers are correct as far as I know.\u00a0 If you find anything that seems incorrect, I would appreciate hearing about it so that I can make corrections. Purpose of Staining: \u00a0increases Contrast & Resolution \u00a0 Provide contrast (easier\u2026","rel":"","context":"Similar post","block_context":{"text":"Similar post","link":""},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/my-all-about-you.com\/wp-content\/uploads\/2018\/07\/srvmicro1.jpg?fit=656%2C343&ssl=1&resize=350%2C200","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/my-all-about-you.com\/wp-content\/uploads\/2018\/07\/srvmicro1.jpg?fit=656%2C343&ssl=1&resize=350%2C200 1x, https:\/\/i0.wp.com\/my-all-about-you.com\/wp-content\/uploads\/2018\/07\/srvmicro1.jpg?fit=656%2C343&ssl=1&resize=525%2C300 1.5x"},"classes":[]},{"id":73,"url":"https:\/\/my-all-about-you.com\/en\/reference\/","url_meta":{"origin":109,"position":1},"title":"The Study","author":"myallaboutyou","date":"January 11, 2018","format":false,"excerpt":"Elegant private library with cherry wood shelves, leather-bound volumes, and a working fire \u2014 a room not for display, but for disciplined thought. The Study is the working room of the house.It is where craft, science, and stewardship meet. Here, knowledge is not collected for admiration but used for preservation.\u2026","rel":"","context":"With 62 comments","block_context":{"text":"With 62 comments","link":"https:\/\/my-all-about-you.com\/en\/reference\/#comments"},"img":{"alt_text":"Elegant private library with cherry wood bookshelves, leather-bound books, rolling ladder, fireplace, and rich traditional furnishings.","src":"https:\/\/i0.wp.com\/my-all-about-you.com\/wp-content\/uploads\/2026\/02\/The-Library-Craft-Care-Living-Tradition.png?fit=800%2C1200&ssl=1&resize=350%2C200","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/my-all-about-you.com\/wp-content\/uploads\/2026\/02\/The-Library-Craft-Care-Living-Tradition.png?fit=800%2C1200&ssl=1&resize=350%2C200 1x, https:\/\/i0.wp.com\/my-all-about-you.com\/wp-content\/uploads\/2026\/02\/The-Library-Craft-Care-Living-Tradition.png?fit=800%2C1200&ssl=1&resize=525%2C300 1.5x, https:\/\/i0.wp.com\/my-all-about-you.com\/wp-content\/uploads\/2026\/02\/The-Library-Craft-Care-Living-Tradition.png?fit=800%2C1200&ssl=1&resize=700%2C400 2x"},"classes":[]},{"id":535,"url":"https:\/\/my-all-about-you.com\/en\/reference\/microbiology-lab-231\/microbiology-lab-231-study-guides\/","url_meta":{"origin":109,"position":2},"title":"Microbiology Lab Study Guides","author":"myallaboutyou","date":"July 21, 2018","format":false,"excerpt":"\u00a0 \u00a0Study Guide Examination 2 Technique used for pure culture isolation? Streak-Plate method Pure Plate method Both involve diluting the bacteria in a sample to an end point where a single cell divides that gives rise to a pure colony. Explain the purpose of staining microorganisms. To identify unknown\/transparent organisms.\u2026","rel":"","context":"Similar post","block_context":{"text":"Similar post","link":""},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/my-all-about-you.com\/wp-content\/uploads\/2018\/07\/bacterial_morphology_diagram-svg-hi.png?resize=350%2C200","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/my-all-about-you.com\/wp-content\/uploads\/2018\/07\/bacterial_morphology_diagram-svg-hi.png?resize=350%2C200 1x, https:\/\/i0.wp.com\/my-all-about-you.com\/wp-content\/uploads\/2018\/07\/bacterial_morphology_diagram-svg-hi.png?resize=525%2C300 1.5x"},"classes":[]},{"id":12818,"url":"https:\/\/my-all-about-you.com\/en\/about\/the-ledger\/formation\/spiritual-discipline\/","url_meta":{"origin":109,"position":3},"title":"Spiritual Discipline","author":"myallaboutyou","date":"February 28, 2026","format":false,"excerpt":"Spiritual discipline is formed through steady practice rather than sudden intensity. The posts gathered here examine habits of prayer, fasting, study, examination, and ordered living as means of spiritual growth. Each reflection considers how small, consistent acts shape faith over time. Select a title below to read the full post.\u2026","rel":"","context":"Similar post","block_context":{"text":"Similar post","link":""},"img":{"alt_text":"Open journal with wooden rosary and cross resting on its pages beside a lit candle and pen on a rustic wooden table in soft natural light.","src":"https:\/\/i0.wp.com\/my-all-about-you.com\/wp-content\/uploads\/2026\/02\/Spiritual-Discipline-%E2%80%93-Habits-of-Prayer-and-Practice.png?fit=1200%2C800&ssl=1&resize=350%2C200","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/my-all-about-you.com\/wp-content\/uploads\/2026\/02\/Spiritual-Discipline-%E2%80%93-Habits-of-Prayer-and-Practice.png?fit=1200%2C800&ssl=1&resize=350%2C200 1x, https:\/\/i0.wp.com\/my-all-about-you.com\/wp-content\/uploads\/2026\/02\/Spiritual-Discipline-%E2%80%93-Habits-of-Prayer-and-Practice.png?fit=1200%2C800&ssl=1&resize=525%2C300 1.5x, https:\/\/i0.wp.com\/my-all-about-you.com\/wp-content\/uploads\/2026\/02\/Spiritual-Discipline-%E2%80%93-Habits-of-Prayer-and-Practice.png?fit=1200%2C800&ssl=1&resize=700%2C400 2x, https:\/\/i0.wp.com\/my-all-about-you.com\/wp-content\/uploads\/2026\/02\/Spiritual-Discipline-%E2%80%93-Habits-of-Prayer-and-Practice.png?fit=1200%2C800&ssl=1&resize=1050%2C600 3x"},"classes":[]},{"id":12862,"url":"https:\/\/my-all-about-you.com\/en\/about\/the-ledger\/formation\/","url_meta":{"origin":109,"position":4},"title":"FORMATION","author":"myallaboutyou","date":"February 28, 2026","format":false,"excerpt":"Becoming, discipline, study, habit, growth. Formation is the intentional shaping of the person as a steward. It is the steady work of aligning daily habits, health, intellect, and vocation with a higher standard of excellence. In this archive, we document the systems of discipline that foster growth \u2014 treating the\u2026","rel":"","context":"Similar post","block_context":{"text":"Similar post","link":""},"img":{"alt_text":"A close-up, naturally lit photograph from a first-person perspective, looking down at a detailed personal workshop bench and study desk made of thick, aged oak wood. The central open notebook with handwritten diagrams is surrounded by an annotated map with Latin, Greek, and French text, a compass, callipers analyzing natural specimens, a miniature mortar and pestle with dried herbs, and a small, framed portrait. Books on 'MEDITERRANEAN TONGUES' and 'ANATOMICAL CURIOSITIES' form a stack. 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Browse by Theme CRAFT The work of hands\u2014wood, fabric, soap, systems, stewardship. 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